IMCB

This post is quite long, take your time!

On Tuesday, we'd Chinese Oral. Aww, the answers are almost the same. I die die simply choose one answer

I didn't go for school for three consecutive days as I'm going for an attachment which I can have the chance to get familiar with zebrafish. I haven't heard of zebrafish before, I'm not sure about you. But it definitely was an eye-opening experience, just like what I had during June holidays.

Basically, this attachment programme was held in A star, biopolis ( somewhere near Buona Vista). It was a fruitful experience I would say.

For the first day, everyone was very excited about zebrafish! Firstly, we collected their embryos from an adult male and an adult female! Next, we placed the embryos into four petri dish with egg water (no bacteria) and subsequently placed them into the 28C incubator to let them develop!

We're divided into 6 bench and each bench were assigned to one teratogen. Our teratogen is ethanol, which is a very very poisonous teratogen to embryos, in other words, it'll kill the embryos! How pathetic. Despite knowing that the zebrafish will die, we need to continue carrying out the experiment!

Starting our teratogen treatment! (please note that they'll throw these fish away at the end of the course as they don't want to affect the ecosystem!) We have three petri dishes and each one is diluted with 100ul, 250ul and 300ul of ethanol respectively. One petri dish, on the other hand, is used as a control. We then use a fine needle to introduce a hole in the chorion of the embryos under a light microscope. This is where we killed some fish because of violence. At the end of the day, we put the petri dishes back into incubator again.




People are so excited and so was I!



This is how zebrafish looks like under light microscope! They're very active!! cute little zebrafish.

On the second day, we dechorionated the zebrafish! Awww, this is awful, I tell you. Dechrionation, a new word for me, anyway, it's disgusting! Most importantly, I killed some zebrafish during this process... I didn't want to, but I accidentally did it. :( Apart from that, we counted the number of zebrafish that died. RIP. This marked the end of second day.

Subsequently, on the third day. we observe the zebrafish again. All fish died, except those in control.... Hmmm, how pathetic. What tells you that die? When you see an opaque egg york, which is like blackish-shit in the middle of the embryos, that tells you they're dead. On the other hand, those which are still alive have translucent egg york, which you can see through clearly.


The zebrafish is almost developed after 60 hours. So, you can see its heartbeat as well as the blood flowing thought their body!

Lesson from this programme, DON'T KILL FISH and DON'T DRINK ALCOHOL WHEN YOU'RE PREGNANT! YOU'LL KILL YOUR BABY!!!

At the end of the programme, the ACS(B) students created a group at facebook called I LOVE ZEBRAFISH. It's time for you to love the nature! They really really need our help.

And.... I made new friends!

It took me such a long time to finish this post.

You can also read related post from Wan Ting's blog! She went for the fruitfly research, which ewww-ed me!

Finally, this post is officially published! huh~